I've not used eagle myself, (kicad, and express pcb straight to printer) but the basic tips are group your components into the main function blocks, do the critical layout and routing in those function blocks, then start tying the blocks together,
this has done me well on even a few of my larger and smaller (stupidly dense 25x25mm) projects, as by keeping the respective blocks tied together there should be less signals needed to be routed to other sections
Run your sensitive or critical traces between these modules first, trying to leave a way to snake out other traces on both sides for later routing.
Lay out your plugs and connectors in logical places, remembering you can still shift these, but it will help to plan your layout.