Just some general things I learned when doing a large step up long ago. First, app notes and online info tends towards low ratio step ups. They can mislead you for large step ups. Odd things can happen with larger ratios and transformer construction may require "tricks" like multiple layered windings and Litz wire. Make sure insulation is sufficient- single build may not be enough. Make sure to measure coupling and such at the frequency of interest. I always had to include a small gap in the transformer to prevent saturation. Remember that saturation isn't a sudden thing, but comes on gradually. Layout is important- keep current loops very short. Even with a ground plane you can have ground loop problems, so single point grounding of some traces may be necessary. Be sure to look at the ESR of input caps along with output caps.