When I posted the other day I was fairly sure that somewhere in my stuff I had some old Russian literature about the use of carnosine (not the patented n-acetyl-carnosine) for cataracts.
One journal article is from Biokhimiya (Moscow) from 2000. I don't have a complete copy, just its abstract handy. But I am pretty sure its on the web, just a bit pressed for time now. (Edit- Link added)
The earlier Russian experience with
l-carnosine eye drops is described in
"MINI-REVIEW: Use of Carnosine as a Natural Anti-senescence Drug for Human Beings" by
A. M. Wang, C. Ma, Z. H. Xie, and F. Shen
PDF version:
http://protein.bio.msu.ru/biokhimiya/contents/v65/pdf/bcm_0869.pdf1
Biochemistry (Moscow), Vol. 65, No. 7, 2000, pp. 869-871. Translated from Biokhimiya, Vol. 65, No. 7, 2000,
Pp. 1022-1024.Original Russian Text Copyright © 2000 by Wang, Ma, Xie, Shen.
Biochemistry (Moscow), Vol. 7 , No. 65 , 2000 , pp. 869-871 . Translated from Biochemistry (Biokhimiya) , 2000 , issue 7
65 volumes, 1022-1024 pages. Original Russian version Copyright © 2000 by Wang (Wang) , Ma (Ma) , Xie (Xie) , Shen (Shen) .
Small comment
Carnosine as a natural anti-aging drug for human use
AM 王 ' *, C. Ma, ZH Xie
'
, and F. Shen
'
Department of Biochemistry, Department of Neurobiology, Harbin Medical University, China 2 , Zip Code 150086
Included on October 29, 1999
Email: Wangam@ems.hrbmu.edu.cn
also
https://ethos.ag/cataracts-wang-chinese.pdfAbstract: Carnosine is an endogenous free radical scavenger. Recent research shows that in addition to preventing cells from being oxidatively induced by stress damage
In addition, carnosine can prolong the life of cultured cells, activate senescent cells, inhibit amyloid peptide (A β ), malondialdehyde, hypochlorous acid
The toxic effects of salt on cells, the inhibition of glycosylation of proteins, and the cross-linking between DNA and proteins and proteins.
And maintain cell homeostasis. At the same time, carnosine can delay the decline of vision caused by aging, effectively preventing and treating aging white
Internal ailments and diseases caused by other ages. Therefore, for humans, carnosine can be used as an anti-aging drug.
Keywords: carnosine, anti-aging drugs
Carnosine is a non-enzymatic free radical scavenger and a natural antioxidant [1, 2]. It is generally distributed in organizations, especially
It is highly concentrated in muscle and brain [3, 4]. Carnosine is produced by Russian scholars Gulewitsch and Amijo
Amiradzibi discovered in 1900 [5], and other biologists such as Severin and
Boldyrev also contributed to the study of the bioavailability and medical applications of carnosine. Overall,
Although the biological properties of carnosine are not as well known as SOD, vitamins C and E, scholars around the world still resist it.
Characteristics, immunomodulator effects, and neuronal free radicals are highly protective [6-8]. Different studies have shown that muscle
Peptides can be used to treat gastric ulcers, burns, hypotension, and anti-brain ischemia/reperfusion injury [1, 9]. The purpose of this article is
This is an experimental study and medical application of carnosine as an anti-aging drug.
Free radicals and aging
Since American scholar Harman [10] pointed out that free radicals may be one of the important factors causing aging and aging diseases.
This theory has gradually been recognized by many scholars around the world.
Many findings have clearly shown that the emergence and development of aging and aging diseases are closely related to cellular damage caused by oxygen free radicals.
Related, it causes cell instability and loss of function, leading to the emergence of aging and aging diseases. Atherosclerosis,
Diseases such as diabetes [11], Alzheimer's disease [12, 13] and aging cataract [14, 15] are associated with free radical damage.
Related. Later studies on the bioavailability of different free radical scavengers and antioxidants have proven that they have a protective
The cells are protected from oxygen free radical damage and normalize cell metabolism. Some well-known antioxidants such as SOD, vitamins
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E, EGB761 and melatonin have been used in medical practice and have achieved good results. So scholars all over the world
Holding the same desire - hope to explore and develop a more effective free radical scavenger to extend life.
Evidence of carnosine anti-aging utility
The bioavailability and medical applications of carnosine have been studied extensively and intensively. In addition to being an antioxidant and free radical scavenging
In addition to the agent, carnosine also activates cell membrane enzymes, inhibits protein non-enzymatic glycosylation and modulates immunoreactivity. The nearest one
These data report that carnosine has the ability to activate senescent cells [16]. Our research in medical applications also shows that carnosine can
To delay the decline of vision caused by aging, while effectively preventing and treating aging cataracts.
In vitro experimental evidence of carnosine anti-aging utility. McFarland and Holliday [16] studied
Effect of carnosine on the growth, morphology and longevity of cultured human diploid fibroblasts. They proved through experiments that
On the Dulbec medium (DMEM), under the action of 50mm carnosine, the shape of the cultured cells is flat, scattered, and the cells
evenly distributed. And these cells grow with this same morphological characteristic throughout their entire life cycle. They found that
Under the action of carnosine, these progeny cultured cells do not exhibit aging morphological features. In this experiment, when the cell population is multiplied
When the number (PDs) is 55.3 and 55.1, that is, when signs of aging appear, the MRC-5 cells are controlled to be transferred to the muscles.
On the medium of the peptide, these cultured cells showed significant signs of activation. These cells continue to grow slowly for 274 days, and
Eventually at the PD level, this is significantly higher than the control group level.
McFrank and Hadley used MRC-5 and HFF-1 cells to detect the efficiency of carnosine for cell ligation and plating [17]
. They observed that on the Dulbec's medium, under the action of 20mm carnosine, carnosine has colony formation on young cells.
The obvious stimulating effect has a more significant effect on the aging cells of the offspring. They also found that carnosine has a significant effect on activated cells.
It stimulates the formation of more flora and has a larger colony area. Among them, the largest flora formed from a single cell is equivalent
15PDs. Through other experiments, it was found that the length of life and the increase in carnosine measurement increased in the same proportion.
McFarland and Hadley observed that under long-term effects, carnosine can promote cell bonding and survival, and long-term
Cells cultured in a medium containing carnosine are more standard than those cultured in a medium that is not injected with carnosine.
Phenotype. Use three different methods (microscopy, flow cytometry, and an ELISA target for DNA oxidative damage)
(Remarks), Kantha et al [18] used mouse embryonic fibroblasts to study L-carnosine pair in nutrient culture.
Study of cell morphology retention effects. Under the microscope, they found that under the same nutrient culture, contrast control
The cells on the medium retain the morphological characteristics of the cells on the 30 mm L-carnopeptide medium, and the cell integrity remains.
All around. By flow cytometry, the proportion of cell damage occurring in the control group was significantly higher than that given 30 mmL-carnosine.
The proportion of fibroblasts in the Burke medium. Viability of fibroblasts on 10mmL-carnosine medium
Characteristics, intermediate levels for the control group and 30 mm L-carnopeptide medium. 8-OH-dG is one of DNA oxidative damage
The main product. According to their report, after five weeks, fibroblasts in the control group showed 8-OH-dG release.
Significant (5 fold) increase in dose (p < 0.05); however, cells cultured on 30 mmL-carnosine medium did not show
This significant increase in 8-OH-dG. These results indicate that in the condition of nutrient culture, in continuous fibroblast culture
, carnosine can maintain cell morphology retention.
Fibrosis of amyloid peptide is an important feature of Alzheimer's disease (AD) [19]. Amyloid peptides are widely recognized and increased
Oxidative stress in cerebral vascular endothelial cells and smooth muscle cells, thus forming peptide accumulation in the AD phase [20], which inhibits endothelial cells
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It replicates and has a direct toxic effect on the peripheral and vascular endothelium. Amyloid peptides in the brain can cause damage to the blood-brain barrier
hurt. Scholars such as Preston et al. [7] studied carnosine pairing by invigorating rat brain vascular endothelial cells (RBE4).
The protective effect of short forms of amyloid peptides on toxic cells. Reduced mitochondrial dehydrogenase activity, lactate dehydrogenase release and
Determination of glucose consumption, they found that the rapid addition of 20mm carnosine before amyloid peptide treatment can effectively prevent 200 and
The toxic effect of 300 mg/ml amyloid peptide on cells. They pointed out that the protective mechanism of carnosine lies in its anti-glycation and anti-oxidation
Activity, during AD, both saccharification and oxidation can cause damage to neuronal nuclear endothelial cells.
Medical applications of carnosine. From our research, we have reported [21, 22] that eye drops containing 20mm carnosine have been
It is used to treat 96 patients aged 60 years with varying degrees of aging cataract, with a morbidity ranging from 2 to 21 years.
The experimental method is that after stopping all other anti-cataract drugs, the patient drops 1-2 drops of the eye drops containing carnosine to each eye.
3-4 times a day, treatment time is 3-6 months. Changes in the degree of visual recovery and changes in lens transmittance for the evaluation of carnosine treatment
Estimate the index. The results showed that for the initial aging cataract patients, carnosine showed a significant effect, the cure rate was 100%.
For patients with late-stage aging cataract, the cure rate of carnosine is 80%. For other types of cataract patients, carnosine is also the same
Shows a good therapeutic effect.
In the observed cases, no side effects were produced. In recent years, we have used the same
The eye drops of the myopeptide content are treated in about 1000 aging cataract patients. These studies have achieved similar results (coming soon
publishing).
In addition, we use myopeptide eye drops to treat patients with varying degrees of visual impairment (no cataract) from 48 to 60 years old.
. The treatment period is 2-6 months. The experimental results show that carnosine can alleviate eye fatigue and improve vision to some extent (If you give a clearer view, you can significantly improve your vision). Patients say that carnosine can brighten and relax their eyes. also It is important to note that the medical applications of these carnosines are of statistical importance.
Therefore, carnosine is a potential drug for the prevention and treatment of aging cataracts and delaying visual aging (a sign of aging). Pharmacology of carnosine
The effect seems to be that it can be immersed in the lens [14, 23], improve the antioxidant capacity of the lens, increase the nano-, potassium-triphosphate
Adenosidase [24, 25] activity regulates metabolism and other physiological responses of the lens. According to reports, in Australia and Russia
Rose, carnosine may be proposed for skin care and wrinkle removal, for nourishing treatment in Germany. Our data also proves the muscle Peptides have anti-aging effects. All of these results can provide valuable data for the use of carnosine as an anti-aging drug.
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It really does amazing things for the eyes. A pet version is available in the US, for around $50 for a 15 ml bottle.
Or you can make it yourself fresh every couple of days using distilled water, a bit of sterile salt, a tiny bit of sodium bicarbonate (baking soda) and a very tiny bit of l-carnosine. It improves vision dramatically and the results are lasting, its not like a drug - the clarifying effect lasts, it doesn't wear off.
Unfortunately, I still have myopia, and some other eye issues. But, Ive had them for a long time. The above paper is true, it does improve vision substantially.