General > General Technical Chat

Working From Home - Impacts of Coronavirus

<< < (263/447) > >>

thinkfat:

--- Quote from: cdev on May 12, 2020, 04:12:18 pm ---You're misunderstanding me completely. I'm sorry. As I sit here I am listening to a Senate committee hearing which is talking about the US's response to COVID-19. I know a lot about functional foods, and have since I was young. I'm trying to help. All ideas with any merit at all should be evaluated. Especially inexpensive, easy to implement  ones. The science is solid. I don't know what else to say. It might help.

--- Quote from: thinkfat on May 12, 2020, 03:40:10 pm ---
--- Quote from: cdev on May 12, 2020, 02:56:34 pm ---It just seems to me like we may be ignoring a substance with great potential to help people.

--- End quote ---

It is of no consequence on the grand scale if the few us here ignore it. I do wonder, very much so, what drives your insistence in this being the right forum for shilling grape seed and red wine.

--- End quote ---

Grapes and wine, as far as I know, even if it did help, I suspect its likely grapes and wine would not contain enough resveratrol to be that useful.

--- End quote ---

"Did help with MERS" - the study you linked is about resveratrol being effective in vitro against the MERS-CoV. It's a bit of a stretch to say it "helped" when it was not clinically tried. The study found that  a certain dosis of the substance prolonged cell culture survival and acknowledges that no in vivo considerations have been made.

cdev:
It seems to me likely that you arent understanding what I'm saying because you ignored what I said and instead youre trying to put words in my mouth.

-------

This is right on topic, and its new. Note that it says that more research is needed.

J Biomol Struct Dyn. 2020 Apr 29:1-16. doi: 10.1080/07391102.2020.1762743. [Epub ahead of print]

Stilbene-based Natural Compounds as Promising Drug Candidates against COVID-19.

Wahedi HM1, Ahmad S2, Abbasi SW1.
https://www.ncbi.nlm.nih.gov/pubmed/32345140

Abstract
The pandemic coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a great threat to public health. Currently, no potent medicine is available to treat COVID-19. Quest for new drugs especially from natural plant sources is an area of immense potential. The current study aimed to repurpose stilbenoid analogs, reported for some other biological activities, against SARS-CoV-2 spike protein and human ACE2 receptor complex for their affinity and stability using molecular dynamics simulation and binding free energy analysis based on molecular docking. Four compounds in total were probed for their binding affinity using molecular docking. All of the compounds showed good affinity (> -7 kcal/mol). However, fifty nanoseconds molecular dynamic simulation in aqueous solution revealed highly stable bound conformation of resveratrol to the viral protein: ACE2 receptor complex. Net free energy of binding using MM-PBSA also affirmed the stability of the resveratrol-protein complex.

Based on the results, we report that stilbene based compounds in general and resveratrol, in particular, can be promising anti-COVID-19 drug candidates acting through disruption of the spike protein. Our findings in this study are promising and call for further in vitro and in vivo testing of stiblenoids, especially resveratrol against the COVID-19.


KEYWORDS:
COVID-19; MM-PBSA; Molecular docking; Molecular dynamic simulations; Stilbene-based natural compounds

PMID: 32345140 DOI: 10.1080/07391102.2020.1762743

---------------------------------

There needs to be research because if it helps, it will be very useful, but it may not help, if thats the case, we need to know that too. People shouldnt try this themselves.  The research community needs to investigate it.

nctnico:

--- Quote from: cdev on May 12, 2020, 06:08:46 pm ---It seems to me likely that you arent understanding what I'm saying because you ignored what I said and instead youre trying to put words in my mouth.

-------

This is right on topic, and its new. Note that it says that more research is needed.

--- End quote ---
Which means: we have no cure or anything remotely useful at all but just wanted our 15 minutes of fame. It seems the daily radically new battery invention hoax has been replaced by a daily radically new Covid-19 cure hoax.

cdev:
Isn't it wise, if somebody is an engineer, to solve each problem by the most straightforward, simple means that exists?


--- Quote from: thinkfat on May 12, 2020, 05:46:05 pm ---"Did help with MERS" - the study you linked is about resveratrol being effective in vitro against the MERS-CoV. It's a bit of a stretch to say it "helped" when it was not clinically tried. The study found that  a certain dosis of the substance prolonged cell culture survival and acknowledges that no in vivo considerations have been made.

--- End quote ---

https://www.ncbi.nlm.nih.gov/pubmed/?term=resveratrol+virus  << Search to get the big picture.


That is what I am saying that we need. Testing in animals. Soon. 

The study with resveratrol, piglets and pseudorabies virus that I cited was to show you that the basic concept is sound, even with a virus that infects the brain. (COVID-19 also infects the brain, and its invasion of the CNS is most likely why people stop breathing. So that paper shows that it can and does kill this other substantially more virulent virus in the piglets' CNS. "in vivo"

 if resveratrol inhibits replication of the COVID-19 virus as its possible it will (see my previous posts) - its time to test it with animal models.

After doing a great deal of reading on the side effects of other drugs being tested, and their results so far, I think there actually is a possibility that - if it works, it may be the best alternative. Because it may help address many other symptoms of COVID-19. Sepsis, pulmonary fibrosis, various coagulopathies, it may be neuroprotective,  nobody knows if it can prevent the propagation of the coronavirus up axons to infect the brain stem. It would be easy to test. )  But it may do much more because of the situation with sepsis and cytokines and chemokines causing sudden changes in the integrityof the respiratory epithelium. Injuring the parts of the lung that do the breathing. Resveratrol helps the lungs recover quite a bit in that situation, acute lung injury and ARDS. It also helps other organs from various kinds of sepsis including polymicrobial sepsis.

There also is a way it could be
aerosolized, like n-acetyl-cysteine has been. Using beta-glucan.

A slight reformulation might make it useful in the lower respiratory tract infection, with its pneumonia.as an aerosol-  it would get the substance to where it would do the most use quickly.

Sometimes pneumonia is caused by injuries that cause a lot of damage, because when cells die they release cytokines. This is also what happens when people suffer any abdominal injury such as blunt force trauma. That causes large amount of cytokie release and the sunbequent injury can become life threatening very quickly. That kind of injury can become an emergency for similar reasons as covid-19 often does even though the cause seems completely different, it really isnt.

From what I have read, resveratrol may prevent the pneumonia devolving into acute lung injury or ARDS.
 in that kind of situation. Its possible that that is what is happening, large numbers of cells may be dying and releasing cytokines/chemokines for a similar reason, cell deaths. If that is the case, resveratrol might be a dramatic help. It may prevent blood clotting and reduce strokes, as it does elsewhere, and reduce the size of infarcts when strokes do occur, as its known to do that. It may be helpful in kawasaki disease coagulopathy in children.

But this is all speculation whether it would in COVID-19.

It MAY be both an antiviral and an immunomodulator in one. That could be a very good thing.

And then again it may not be. But it seems it has potentials that none of the other proposed therapies have, and more of a track record as far as we already know it may have use in some of its symptoms, when caused by other (biologically diverse) pathogens including viruses. Whether it would help with them is a long shot, but you can do this yourself, investigate on pubmed if various COVID-19 pathologies return any results when you add the word resveratrol. But this investigation needs to be done in animal models, not people.

And let me make it clear here, I'm not a doctor, I am just speculating here, and its a long shot because there are so many variables. But I would be lying if I didnt say that it seems as if there MAY be a path to a unique solution to this huge problem - and it just seems worth trying. If it works, and again, many IFs exist, but IF it works in its simplest form, it could be a huge help, even if it simply lowered the severity of the infection a small amount, because by starting sooner, even low dose resveratrol (which is actually a food, not a drug) might  help keep a significant number of infected people out of the hospital and ICU.

thinkfat:
I repeat: this is the wrong place.

PS:

--- Quote ---Wahedi HM1, Ahmad S2, Abbasi SW1.
https://www.ncbi.nlm.nih.gov/pubmed/32345140

--- End quote ---

Full text is here: https://www.tandfonline.com/doi/full/10.1080/07391102.2020.1762743

You must be kidding: This is not even in vitro. This is a molecular simulation study.

Navigation

[0] Message Index

[#] Next page

[*] Previous page

There was an error while thanking
Thanking...
Go to full version
Powered by SMFPacks Advanced Attachments Uploader Mod