Some healthy skepticism has been raised on this forum regarding the Lishtot TestDrop Pro and its method of performance. This post is to help explain how the device works and the science behinds its design.
Water has been shown to be a triboelectric material, namely it can deliver electrons (primarily from hydroxide ion, it would appear) to a wide range of materials. Below are links to two academic papers and one US patent that are based on the triboelectric behavior of water with plastic materials. It is noted in the first reference that water would be only less positive than air in a proper ranking of materials in a standard triboelectric series.
https://www.sciencedirect.com/science/article/abs/pii/S030438861630002Xhttps://www.sciencedirect.com/science/article/abs/pii/S0304388611001100https://patents.google.com/patent/US4594553A/enAs noted in the above references, water imparts a charge on plastic materials by virtue of contact between water and the same, just as a person gets charged up by walking on a rug in the winter. The static charge deposited on the plastic has associated with it an electric field. The prior art work cited above measured the charge delivered to the plastic generally with two electrodes.
One important outcome from the second reference was the inadvertent realization that the size of the field associated with charge delivered would be related to what was present in the water. The graphs below are from the Ravelo paper:
Note the Y-axis labels. The distilled water has a range from 0-250 mV, while the "ordinary water" has a scale of 0-40 mV. The Ravelo paper was the first to give an inkling that the amount of charge transferred from water to a plastic material was directly related to what was present in the water, or in other words, water quality.
The TestDrop Pro currently has three US patents associated with it:
https://patents.google.com/patent/US9453810B2/https://patents.google.com/patent/US10240182B2/https://patents.google.com/patent/US10060895B2/The basic function of the device is to run an unpowered electrode through the electric field generated on a plastic surface by electrons delivered from water. The device makes 100 measurements per second and by moving one device towards a cup with water, we can get a large number of data points in only a few seconds. As one would expect, as the device gets closer to a cup with water, the electric field becomes stronger. Below is a canonical graph of "clean water". Note that as the device moves towards the cup (represented by time in seconds on the X-axis), the field becomes more and more negative.
This result is completely consistent with the data from the three prior art publications cited above. Water is a triboelectric material. When contacted to a plastic surface, it transfers electrons to the plastic. A static electric charge will have an electric field associated with it. An unpowered electrode moving towards a source of static electricity will record stronger and stronger negative electric fields as it approaches the charged-up plastic.
So we can make a measurement that is related to water's interaction with plastic. This interaction has been seen with a wide range of plastic materials in different formats such as cups, bottles, pipette tips, disposable pipettes, etc. So how does one get discrimination? Or more accurately, why would water samples give different signals that allow for identification of contaminated samples?
The TestDrop Pro is trained to identify clean water samples, namely those that have profiles similar to the one shown above. One can program the device for different levels of sensitivity but the general concept is that clean water allows for a lot of charge to be transferred to a plastic container or material. When water is contaminated, fewer electrons reach the plastic. Why? Essentially, there appear to be two different reasons:
1. Inorganic contaminants such as heavy metals appear to coordinate hydroxide and make electron release to plastic more difficult.
2. Organic/biological contaminants bind to plastic near the waterline and due to their association with water via hydration and/or hydrogen bonding effectively extend the waterline higher up in the cup or container.
The current protocol for the TestDrop Pro has measurement of electric field in the open space over the waterline. The reasoning is that this is where one should fiend lots of electrons transferred by water after swirling, without interference of the now positive water and any contributions from whatever its contents may be. If fewer electrons are transferred to the plastic, the resulting fields are weaker, as shown in the graph below for a sample that included sodium hypochlorite ("bleach") and ppb levels of lead as free divalent ion:
The algorithm associated with TestDrop Pro is designed to look for a certain percentage of a graph below a predetermined threshold. In the above graph, the lead/chlorine sample does not pass that threshold and the sample would be flagged as contaminated.
As mentioned above, the water, missing electrons left on the plastic, becomes positively charged due to unbalanced hydronium ions (hydroxyl radical is neutral). Below is a graph of approaching the cup, this time below the waterline:
Organic/biological samples tend to wipe out signal until the concentration of the contaminant is very low, ppb or less. Below is a graph for suspected carcinogen PFOS in tap water.
The performance of the device as a screen for inorganic and organic contaminants allows for rapid determination of whether a water sample needs further laboratory attention. Below are links to testing performed outside of Lishtot's labs with the TestDrop Pro. We were not involved in any of the testing reported by Analiza or MIT. Additional companies and research labs have performed research under nondisclosure agreements.
https://www.lishtot.com/reports/180108 Lishtot TestDrop Pro - PFOS - FINAL.pdfhttps://www.lishtot.com/reports/180105 Lishtot TestDrop Pro - Protein - FINAL - AB AK Signed.pdfhttps://www.lishtot.com/LishtotAnalysis_Nepal_Experimental_Plan-Lishtot_TestDropPro_Jan201For additional information, please visit
https://www.lishtot.com/