Impossible to tell, with that amount of information. Does the circuit "just" have work or do you need to pass EMC tests?
Just a few ideas:
- I wouldn't be too concerned about the split, since the signals have a solid GND plane as reference (assuming the signals are launched between the signal line and GND with no return path discontinuities somewhere else). It's always better to have the signals closer to GND than to the power planes, of course.
- It's almost always a good idea too slow down the digital edges as much as possible. Just don't forget about the I2C falling edges.
- What's on layer 5? If layer 5 isn't a ground plane, a significant amount of the circuit's power supply energy is stored between Layer 2 and 4. Just where the digital signals happen to be. So you might see power supply noise on your signals, or signal noise on your power supply.
As I said, it's hard to tell. The circuit could work perfectly well, even in a EMC test or exactly the opposite
But I would be more concerned about the signals being in the space where the power comes from than about the split in the plane.