I'd keep 20 mil spacing (0.5mm) in the power supply section, around the higher voltage stuff. This is overkill for 24V. Everywhere else, you are more than safe to use the fab's minimum width/space rules, which are usually 6/6 mil (0.1524mm).
Mind the trace width for power, and ground if applicable. And also trace length, since you'll have a lot of power running back and forth, and you don't want much voltage drop.
Ground should be filled on both sides, so current capacity shouldn't matter much, but do make sure to inspect that a thick and very redundant ground path is available to all components. Power should be preferentially routed around the heaviest loads first (which I guess will be your LEDs and plug-in dongles, IIRC?). Don't forget bypasses every inch or two (not necessary per chip, but you don't want too much distance between, and again, make sure there is a clear path for ground as well).
Tim