The good news is the ISP tool works.
The HC32L110 board I am using

Wiring:
* RTS -> P00
* RX -> P31
* TX -> P27
* GND -> GND
* 3.3V -> VCC
1. Switch the language to English.

2. Target MCU: HC32L110x4xx
3. Com Baud: 115200 (691200 always fails on my CH340)
4. Hex File: you need provide some dummy hex file, it's required but you can choose to erase without uploading
5. COM Setting: the port used by USB2TTL
6. Check Erase + Blank Check, leave Program,Verify,Encrypt unchecked. If you just want to test the communication without erase, uncheck them all.
7. Click Execute

Then it will reset the chip, do the erase and blank check. The output log tells the result of each step, 成功 means success.
The tests without connecting RTS to P001. Using reset button
If I click Execute and press RESET at the same time, at a 20% success rate it successfully connected to the board and did the erase.
2. Disconnect/Connect VCC manually
I tried to reconnect VCC and click Execute button at the same time, I tried many times with no luck. In theory there is a possibility but the time window may be too short.