
For reflected light microscopes you usually use special objectives (right). Olympus marks these objectives with an M (“metallurgical”). The more common “biological” objectives (left) are designed to be used with a coverglass. Metallurgical lenses, on the other hand, give you the best pictures without a coverglass. With small numerical apertures, but the influence of the coverglass is negligible. However, there is another important difference between the two lens types. The lenses in metallurgical objectives are usually additionally coated so that they reflect less light. This is very important for coaxial illumination, where the illumination of the object follows the same path as the light reflected from the object.
The two lenses above are broadly comparable. They differ only minimally in terms of their magnification and numerical aperture. Both lenses are the revised U variants.

For comparison, the same black plastic surface was photographed with both lenses. The white balance was fixed, only a small adjustment of the exposure time was made. The images have not been post-processed. The biological lens clearly shows a bright spot in the inner area. The shorter wavelengths are more noticeable. The metallurgical objective, on the other hand, shows no weaknesses.

The weaknesses are not overly noticeable on a detailed picture.

The difference becomes somewhat clearer if the color information of the images is discarded and the contrast is greatly increased.

Due to the different numerical aperture, it is difficult to compare the resolution of the objectives. However, the image quality of the metallurgical objective appears better than one would expect due to the slightly higher numerical aperture.
https://www.richis-lab.de/Howto_Microscope_MObjectives.htm 