No, the glass isn't darkened. I'd replace them immediately in that case. Now that I've thought about it for a bit, I'll probably just end up replacing them anyway, they're cheap as hell and the things they protect are not. I've got a dozen or so of the "standard" ones. There's one weird fat one; either I'll leave it alone, or I'll test to see when it breaks down and try to find one that matches. Too bad they don't have part numbers...

Assuming they haven't already failed, I don't think they operate at all during "normal" function. I haven't played around with this all that much, but the only glow it gives off is from the tube heaters. I haven't gotten around to mapping out the PCB yet, so I'm not 100% sure how the circuit works. They may strike during transient events, or maybe they're just failsafe components in case something else doesn't operate correctly (the tubes become leaky and arc over?).
Edit: Just re-read your post. If the neons are being used as indicator lights, they will eventually wear out, refer back to the article I quoted and look at operational hours in the data sheets, some are as low as 2000 hours
Ah, I don't care about the indicators. They don't allow things to break when they fail, I can just replace them when they stop lighting up. Maybe in a more professional environment it'd be important to make sure the HV warning lamp lights when the HV is turned on, but at home it's just me, and I can just look at the switch...