Yes, but it'll help to know more about your design. What kinds of circuitry are you using (ie. analogue, RF, digital or power management), how big is the board, how much power does it use, what's the nature of the problem you're seeing?
Without specifics, you might get a lot of generic advice, but little or none of it could be applicable to your actual PCB.
A good start is to post your schematic and some gerbers or PDFs of the board. Can you do that?