The VFD can be removed using a desoldering tool with care, it's obviously a tedious task but reasonably safe.
This would be the last thing I would like to do and only if absolutely necessary. After all the machine seems to be working well, only with a display problem.
If your device uses an off-VFD driver chip, then higher voltages will obviously be present and contamination on the circuit board could potentially cause this symptom. Easy enough to clean up the board and see if it makes a difference.
There is 32V DC voltage present on the board, supplied through the black wire of the interface cable. On the photo of the back of the board, it's the thin trace on the left edge of the board. It looks like the designer discreetly minimised its interference with other signals.
At first, I would check the signals on the interface cable if there is any sign of life when you would expect the display to change.
If so, the suspicion narrows down to the actual display control.
Without removing the VFD tube you won’t be able to inspect what is hidden beneath.
The communication interface can be anything from 4/8 bit parallel to SPI or even UART.
Good point. Having had another look at the interface cable, there are several wires the functions of which I now know. Starting from the Black (1): 1: 32V DC (this should be for the VFD anode); 2: 5V - this is probably for one or more logic IC on this board (under the VFD); 3: GND; 7: NC; 11/12: 4.5V AC for filament. There are only 6 wires left for signals.
Probed some of these 6 wires with a scope, but didn't get a clue yet.
Not I think the problem is very likely with those chips underneath. It's unlikely for the detail of display segments to be determined by the MCU(s) on the main board and signal transferred through this 12-pin cable. Very unlikely. And it's very likely the 40-pin IC is an MCU for the VFD (and potentially for the buttons and LEDs). Now my guess is that the two 16-pin's are logic ICs. The culprit is probably something around them or one of these two ICs themselves.
It looks like now I have a hard decision to make, whether to desolder the VFD module, risking making things worse. (This would not be a hard one if I had a desoldering station that can suck when the head is on heating, such as the one used by Mr Carlson's Lab - this desoldering job would be a piece of cake!)
But among the things that should be tried before this move, I will replace all the 22uF 6.3V electrolyte caps (and another 1uF 63V) first.