I know what you mean

If you want to measure a cable, I wouldn't use 2 cables for calibration.
I would calibrate with one cable for OSL & connect that same cable to the second port for a through.
That way your measurement plane will be at the connector of the second port, to which you connect DUT cable to test.
Idealy you would not use any gender adapters, but even if you do, they should not introduce too much loss (if they are from a reputable manufacturer).
And the adapter loss should be stated in the datasheet.
In my experience, N adapters have a loss of 0.1db to 0.4db depending on frequency (up to 3GHz).