Hi,
You need to study the document:
https://sites.bu.edu/engcourses/files/2016/08/mosfet-differential-amplifier.pdfthat you provided in the first message.
It shows:

The author has chosen the set the current in MA at 20uA. I chose 10uA.
In the pdf document the author has indicated W/L ratios for the FETs.
In the model that you provided, and I edited, all the W/L ratios are the same.
Io sets the operating currents. These are mirrored using MA, MB and MC. In the authors example MB is operating at 1/5 of the current of MA. It is 1/5 because of W/L ratio.
M3 and M4 are the current mirror load for the differential pair M1 and M2
E2 is a voltage-controlled voltage source. This is used to provides feedback. I implemented this as a simple resistive divider, E2 = 0.5 x Vout.
Jay_Diddy_B