Yes, you can connect to ground any of secondary output, to have ground referenced output (output positive or negative to ground). But, I'm doubting that is enough for driving X-ray tube. Perhaps some sort of output current and voltage control should be necessary for prolonged tube lifetime. Maybe you should consult tube datasheet/manufacturer to see at least what is min. voltage (different anode needs different voltage, e.g. tungsten usually needs 60 kV, some other works starting with just 15-20 kV, etc.). There you can also get some idea about grounding.