What do u mean by Isolate? I mean ground is ground?
2 layer board
No, ground isn't ground. (There's a reason there are different symbols for earth ground, chassis ground, signal ground, analog ground, digital ground, etc.: so that they can be used separately within a circuit.) Current flows through ground, like any other wire or trace, and sometimes you want different parts of the circuit on "separate" grounds -- yes, they'll all connect somewhere, but the idea is that almost no current ever flows through that one spot.
Take a look at
https://www.onelectrontech.com/pcb-layout-design-tips-grounding-considerations/ -- it explains it quite well.
Your PCB layout really isn't very good. I fear your power traces (VCC) may be much too thin, contributing to the problem. But also, all the caps on VCC are too far away from the ICs -- they should be located literally as close as possible, with as fat traces as possible. On your board, they meander around, creating loops that can induce currents in other traces, potentially resulting in noise and instability.
I also see that many signal traces take very... creative paths. Like the output of RV5 (subwoofer out), which starts on the top layer, goes down to the bottom layer (through a via right next to the through-hole pin it came from!
), then goes back to the top, then back to the bottom, back to the top, back to the bottom again, and finally back to the top to connect to C153. That literally could have been accomplished with a single via, by having it come out of RV5 on the bottom, where it has a clear shot all the way over to near C153, where it would pop back up to connect. (And with careful layout, it likely could have been routed entirely on the top, with other signals traveling beneath C153, for example.
Was this autorouted? Audio is something where autorouting really isn't wise, in that you often have both sensitive signal paths as well as heavy power paths right near each other.
I'm expecting my hand-routed TPA3126 PCBs from China this week. I'm of course rather curious as to how well they'll perform. I'm sure I've made some layout mistake somewhere, but I think all the important bits got covered.