I'm not sure if you fully realize that if you connect it to USB, it no longer will be floating if laptop or PC is connected to mains socket, and how huge of a detriment it is. High chance to blow up DUT, uCurrent and computer too. It would be nowhere as easy and safe to use as one connected to a battery powered multimeter. Not to say USB is a very significant noise source which may be an issue when you measure tiny currents. Also dual channel means these channels will not be isolated, which again very severely limits its use and more so increases chance to blow up things. You could use USB isolator but it won't fix the issue with channel isolation. You could implement isolation in the meter part, but then it would severely increase complexity. IMHO the best option would be to build two single channel devices each using USB isolator. Or if you do it with virtual COM port, then you could isolate UART using optocouplers or cheap digital isolator.
Okay, a lot to digest here...
So, currently, I use two separate circuits, each powered by a 2032 coin cell. Currently both of these circuits are connected to an MCU which is used to measure the voltage on the ucurrent circuit.
Is this, in and of itself, a risky setup if I am not using any sort of USB isolator between the MCU board and the PC and the PC is connected to mains power?
If yes, and if I understand correctly you are suggesting that I really should be using two separate MCUs which each use a USB isolator when connecting to the PC.
It seems like generating the +/- 2.5 V that I need wouldn't necessarily be that difficult but that that's the least of the concerns in terms of creating robust setup.
I could also go another route and just try to find a better battery option than using disposeable coin cells. I'm thinking that maybe if I was able to find a rechargeable battery pack in the range of 4.5 V to 5 V that that might be a better option. It would still run on battery when being used but it would get me higher voltage and be more convenient then needing to replace coin cell batteries.